Question 1

Often natural disasters leave little intact DNA with which to identify the victim's remains using traditional DNA fingerprinting techniques.The discovery of ______ made genetic analysis possible with minute amounts of genetic material.

Accepted Answer

A)  miRNAs
B)  frameshift mutations
C)  SNPs
D)  microarraysE) None of the above
F) All of the above

Question 2

Geneticists can create sequences of DNA from RNA using enzymes called ______.

Accepted Answer

A)  palindromes
B)  reverse transcriptases
C)  restriction endonucleases
D)  ligases
E)  DNA polymerasesF) A) and B)
G) B) and D)

Question 3

The field of pharmacogenomics uses knowledge of an individual's single nucleotide polymorphisms to determine how they will respond to a particular drug.

Accepted Answer

A) True 
 B)False

Question 4

Which of the following is a promising treatment for stopping the expression of an unwanted gene?

Accepted Answer

A)  DNA fingerprinting
B)  Antisense therapy
C)  Gene therapy
D)  CloningE) A) and D)
F) A) and C)

Question 5

Your infant has been diagnosed with severe combined immunodeficiency disease (SCID) .Your doctor suggests which treatment as a possible cure for the disease?

Accepted Answer

A)  Genome mapping
B)  Reverse transcription
C)  Gene therapy
D)  MicroarraysE) None of the above
F) C) and D)

Question 6

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

A)  genes
B)  codons
C)  base pairs
D)  proteins
E)  tripletsF) None of the above
G) A) and E)

Question 7

Transgenic organisms are ______.

Accepted Answer

A)  created in nature
B)  only microorganisms
C)  copyrighted
D)  patentedE) A) and B)
F) All of the above

Question 8

Transgenic animals are referred to as _____ modified organisms.

Accepted Answer

A)  chemically
B)  physically
C)  naturally
D)  geneticallyE) C) and D)
F) A) and D)

Question 9

Which step in gene mapping can occur even more rapidly by PCR?

Accepted Answer

A)  Target DNA removed from cells and isolated
B)  Cloning host treated with calcium chloride and receives plasmid
C)  Target DNA and plasmid treated with the same restriction endonuclease
D)  Desired protein is produced by cloning host
E)  Gene is amplified by multiplication of cloning hostF) A) and B)
G) A) and C)

Question 10

EcoRI and HindIII are ______.

Accepted Answer

A)  palindromes
B)  reverse transcriptases
C)  restriction endonucleases
D)  ligases
E)  DNA polymerasesF) A) and E)
G) B) and E)

Question 11

It is possible to identify mRNA molecules using fluorescently labeled cDNA.

Accepted Answer

A) True 
 B)False

Question 12

The difference between somatic cell gene therapy and germline therapy is that

Accepted Answer

A)  germline therapy overcomes a protein malfunction in specific tissues but is not repaired in the entire organism and cannot be passed on to offspring.
B)  somatic cell gene therapy is introduced into the egg, sperm or developing embryo, whereas germline therapy introduces a new gene into a mature tissue.
C)  somatic cell gene therapy overcomes a protein malfunction in specific tissues but is not repaired in the entire organism and cannot be passed on to offspring.
D)  germline therapy is the temporary repair of a genetic mutation, whereas somatic cell therapy is a permanent fix.E) All of the above
F) C) and D)

Question 13

One reason the field of proteomics is continually evolving following the systematic study of an organism's genome is because

Accepted Answer

A)  it became apparent that while the organism's genome is relatively stable, protein expression in an organism is constantly changing.
B)  the expression of proteins is fixed in each organism so sequencing the genome is essential to understanding phenotypes.
C)  it became apparent that protein expression is relatively uniform, whereas the genome is constantly changing.
D)  once the genome had been sequenced, all protein expression was understood.E) A) and B)
F) C) and D)

Question 14

Which step in gene mapping involves transformation?

Accepted Answer

A)  Target DNA removed from cells and isolated
B)  Cloning host receives plasmid
C)  Target DNA and plasmid treated with the same restriction endonuclease
D)  Desired protein is produced by cloning host
E)  Gene is amplified by multiplication of cloning hostF) C) and D)
G) None of the above

Question 15

When DNA is heated, the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 16

The primers in PCR are

Accepted Answer

A)  synthetic DNA oligonucleotides.
B)  reverse transcriptases.
C)  bacterial enzymes.
D)  DNA polymerases.
E)  short RNA strands.F) B) and C)
G) None of the above

Question 17

The advantage of germline therapy over somatic cell therapy is that the normal gene is inserted into an egg, sperm, or developing embryo so that the repair is present in every cell of the organism as it matures to adulthood.

Accepted Answer

A) True 
 B)False

Question 18

Identification of unique DNA fingerprints relies on the presence of single nucleotide polymorphisms among samples.

Accepted Answer

A) True 
 B)False

Question 19

It is now possible to very quickly map the genome of an organism or virus.

Accepted Answer

A) True 
 B)False

Question 20

Analysis of DNA fragments in gel electrophoresis is based on

Accepted Answer

A)  larger fragments moving slowly and remaining closer to the wells.
B)  DNA having an overall negative charge and moving to the positive pole.
C)  DNA fragments being stained so that they can be seen.
D)  application of an electric current through the gel causing DNA fragments to migrate.
E)  All of the choices are correct.F) A) and B)
G) A) and C)

Exam 10: Genetic Engineering and Recombinant Dna

The advantage of germline therapy over somatic cell therapy is that the normal gene is inserted into an egg, sperm, or developing embryo so that the repair is present in every cell of the organism as it matures to adulthood.

Correct AnswerverifiedShow Answer

When DNA is heated, the two strands will separate.

Correct AnswerverifiedShow Answer

It is now possible to very quickly map the genome of an organism or virus.

Correct AnswerverifiedShow Answer

Transgenic animals are referred to as _____ modified organisms.

Correct AnswerverifiedShow Answer

Which step in gene mapping involves transformation?

Correct AnswerverifiedShow Answer

EcoRI and HindIII are ______.

Correct AnswerverifiedShow Answer

Analysis of DNA fragments in gel electrophoresis is based on

Correct AnswerverifiedShow Answer

The field of pharmacogenomics uses knowledge of an individual's single nucleotide polymorphisms to determine how they will respond to a particular drug.

Correct AnswerverifiedShow Answer

Which of the following is a promising treatment for stopping the expression of an unwanted gene?

Correct AnswerverifiedShow Answer

It is possible to identify mRNA molecules using fluorescently labeled cDNA.

Correct AnswerverifiedShow Answer

The size of DNA is often given in the number of _____ that it contains.

Correct AnswerverifiedShow Answer

Geneticists can create sequences of DNA from RNA using enzymes called ______.

Correct AnswerverifiedShow Answer

Your infant has been diagnosed with severe combined immunodeficiency disease (SCID) .Your doctor suggests which treatment as a possible cure for the disease?

Correct AnswerverifiedShow Answer

Identification of unique DNA fingerprints relies on the presence of single nucleotide polymorphisms among samples.

Correct AnswerverifiedShow Answer

Which step in gene mapping can occur even more rapidly by PCR?

Correct AnswerverifiedShow Answer

Transgenic organisms are ______.

Correct AnswerverifiedShow Answer

The primers in PCR are

Correct AnswerverifiedShow Answer

One reason the field of proteomics is continually evolving following the systematic study of an organism's genome is because

Correct AnswerverifiedShow Answer

The difference between somatic cell gene therapy and germline therapy is that

Correct AnswerverifiedShow Answer

Often natural disasters leave little intact DNA with which to identify the victim's remains using traditional DNA fingerprinting techniques.The discovery of ______ made genetic analysis possible with minute amounts of genetic material.

Correct AnswerverifiedShow Answer

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