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Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis

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Question 41

Multiple Choice

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Bacteria that require special growth factors and complex nutrients are termed

A) aerobic.
B) anaerobic.
C) fastidious.
D) microaerophilic.
E) autotrophic.

F) D) and E)
G) All of the above

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Question 42

Multiple Choice

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Which type of microscope shows cells against a bright background but also differentiates intracellular structures of unstained cells based on their varying densities?

A) bright field
B) dark field
C) phase contrast
D) differential interference
E) electron

F) C) and D)
G) B) and C)

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Question 43

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A differential interference contrast microscope uses dyes to give colored three-dimensional images.

Basic dyes are

Bacterial cultures are easily identified from their microscopic appearance.

Please read the clinical scenario, and then answer the questions that follow to become familiar with the traditional NCLEX question format. Ms. Hungh, a Burmese immigrant, enters your clinic with her interpreter complaining of fatigue, weight loss, persistent cough, and rust-colored sputum. The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking assistance for her condition. -Ms.Hungh's acid-fast stain results returned inconclusive for the presence of AFBs (acid-fast bacilli) .Therefore,culturing of the sputum is performed in order to isolate microbial growth for further analysis.Which medium would be utilized to select for the growth of Mycobacterium species if present in the sample?

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is

The _____ of the microscope holds and allows selection of the objective lenses.

The Five I's of studying microorganisms include all of the following except

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. Mr. Nowak is a homeless patient who presented to your clinic today with an injury to his left arm. He injured it on an old piece of scrap metal. His wound is red, hot, and tender with purulent drainage. You swab the site and send it to the laboratory for microbial analysis. -Mr.Nowak's lab results return positive for Staphylococcus.You understand that the culture most likely required growth on a complex medium,consisting of

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. You begin your shift at the inner city outreach clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. Her complaint is that she woke this morning leaking clear to milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, and explain that a vaginal speculum exam will be performed and specimens will be taken for examination. Once obtaining the proper specimens, you label them appropriately, and send the wet mount and culturette to the laboratory for processing. -When utilizing a light microscope,the specimen on the glass slide must be in proper position to ensure illumination of the specimen for visualization.This is done by placing the glass slide

A reducing medium contains

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?